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phoenix eco retroviral ecotropic packaging cell line  (ATCC)


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    ATCC phoenix eco retroviral ecotropic packaging cell line
    Phoenix Eco Retroviral Ecotropic Packaging Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phoenix+eco+retroviral+ecotropic+packaging+cell+line/Phoenix-ECO/pmc10154250__41590_2023_1477_MOESM1_ESM-39-1-20
    Average 97 stars, based on 393 article reviews
    phoenix eco retroviral ecotropic packaging cell line - by Bioz Stars, 2026-11
    97/100 stars

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    Retroviral:

    Article Title: Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 + T cells.
    Article Snippet: B16-F10 (CRL-6475) tumor cell lines were purchased from the American Type Culture Collection and transduced with a retrovirus to express ovalbumin (B16-OVA). .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney cells was obtained from the American Type Culture Collection (CRL-3214) and maintained in RPMI 1640-Glutamax medium supplemented with 10% heat-inactivated FBS, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin sulfate. .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney cells was obtained from the American Type Culture Collection (CRL-3214) and maintained in RPMI 1640-Glutamax medium supplemented with 10% heat-inactivated FBS, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin sulfate.

    Article Title: Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 + T cells
    Article Snippet: .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney cells was obtained from the American Type Culture Collection (CRL-3214) and maintained in RPMI 1640-Glutamax medium supplemented with 10% heat-inactivated FBS, 100 U ml −1 penicillin and 100 μg ml −1 streptomycin sulfate. ..

    Article Title:
    Article Snippet: .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney (HEK) cells was obtained from ATCC (CRL-3214) and maintained in RPMI 1640-Glutamax media supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin and 100 g/mL streptomycin sulfate. .. Authentication Cell lines were not authenticated after purchase from ATCC Mycoplasma contamination All cell lines were tested for mycoplasma contamination prior to use in experiments Commonly misidentified lines (See ICLAC register) NA Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals 6 weeks-old female C57BL/6 mice aged were purchased from Harlan (Harlan, Netherlands) and housed at the animal facility at the University of Lausanne in compliance with guidelines.

    Article Title: Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 + T cells.
    Article Snippet: B16-F10 (CRL-6475) tumor cell lines was purchased from ATCC and retroviral transduced to express ovalbumin (B16-OVA). .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney (HEK) cells was obtained from ATCC (CRL-3214) and maintained in RPMI 1640-Glutamax media supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin and 100 g/mL streptomycin sulfate. .. Authentication Cell lines were not authenticated after purchase from ATCC Mycoplasma contamination All cell lines were tested for mycoplasma contamination prior to use in experiments Commonly misidentified lines (See ICLAC register) NA Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals 6 weeks-old female C57BL/6 mice aged were purchased from Harlan (Harlan, Netherlands) and housed at the animal facility at the University of Lausanne in compliance with guidelines.

    Derivative Assay:

    Article Title: Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 + T cells.
    Article Snippet: B16-F10 (CRL-6475) tumor cell lines were purchased from the American Type Culture Collection and transduced with a retrovirus to express ovalbumin (B16-OVA). .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney cells was obtained from the American Type Culture Collection (CRL-3214) and maintained in RPMI 1640-Glutamax medium supplemented with 10% heat-inactivated FBS, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin sulfate. .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney cells was obtained from the American Type Culture Collection (CRL-3214) and maintained in RPMI 1640-Glutamax medium supplemented with 10% heat-inactivated FBS, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin sulfate.

    Article Title: Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 + T cells
    Article Snippet: .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney cells was obtained from the American Type Culture Collection (CRL-3214) and maintained in RPMI 1640-Glutamax medium supplemented with 10% heat-inactivated FBS, 100 U ml −1 penicillin and 100 μg ml −1 streptomycin sulfate. ..

    Article Title:
    Article Snippet: .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney (HEK) cells was obtained from ATCC (CRL-3214) and maintained in RPMI 1640-Glutamax media supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin and 100 g/mL streptomycin sulfate. .. Authentication Cell lines were not authenticated after purchase from ATCC Mycoplasma contamination All cell lines were tested for mycoplasma contamination prior to use in experiments Commonly misidentified lines (See ICLAC register) NA Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals 6 weeks-old female C57BL/6 mice aged were purchased from Harlan (Harlan, Netherlands) and housed at the animal facility at the University of Lausanne in compliance with guidelines.

    Article Title: Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 + T cells.
    Article Snippet: B16-F10 (CRL-6475) tumor cell lines was purchased from ATCC and retroviral transduced to express ovalbumin (B16-OVA). .. The Phoenix Eco retroviral ecotropic packaging cell line, derived from immortalized normal human embryonic kidney (HEK) cells was obtained from ATCC (CRL-3214) and maintained in RPMI 1640-Glutamax media supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin and 100 g/mL streptomycin sulfate. .. Authentication Cell lines were not authenticated after purchase from ATCC Mycoplasma contamination All cell lines were tested for mycoplasma contamination prior to use in experiments Commonly misidentified lines (See ICLAC register) NA Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals 6 weeks-old female C57BL/6 mice aged were purchased from Harlan (Harlan, Netherlands) and housed at the animal facility at the University of Lausanne in compliance with guidelines.

    other:

    Article Title: Orthogonal cytokine engineering enables novel synthetic effector states escaping canonical exhaustion in tumor-rejecting CD8 + T cells.
    Article Snippet: Authentication Cell lines were not authenticated after purchase from ATCC Mycoplasma contamination All cell lines were tested for mycoplasma contamination prior to use in experiments Commonly misidentified lines (See ICLAC register) NA Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals 6 weeks-old female C57BL/6 mice aged were purchased from Harlan (Harlan, Netherlands) and housed at the animal facility at the University of Lausanne in compliance with guidelines.



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    phoenix eco retroviral ecotropic packaging cell line  (ATCC)
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    Figure 2. Functionality of BCMA-specific CAR expressing shRNA CD3ζ. (A) Schematic representation of BCMA-specific CAR-shRNA CD3ζ genetic constructs. CAR, chimeric antigen receptor; LTR, long terminal repeat sequence; ψ, <t>retroviral</t> Psi packaging element; 2A, 2A self-cleaving peptides; shRNA, short hairpin ribonucleic acid; tCD34, truncated CD34; UTR, untranslated regions. (B) Percentage of transduced CD34+ cells (left) or BCMA CAR+ cells (right) among living cells at harvest (day 10) of CAR T-cells generated from 7 healthy donors measured by flow cytometry. (C) IFN-γ secretion in the supernatant upon 24 h co-culture of CAR T-cells generated from 7 healthy donors with multiple myeloma cancer cell line RPMI-8226 (1:1 ratio), as measured by ELISA. (D) Cytotoxic activity was measured using an LDH assay in the supernatant of 24 h co-cultures of CAR T-cells generated from 7 healthy donors with multiple myeloma cancer cell line RPMI-8226 (1:1 ratio). (E) IFN-γ release of BCMA-specific CAR generated from 3 healthy donors in an RPMI-8226 co-culture with or without soluble recombinant BCMA-Fc protein (R&D Systems, 5 µg/mL). Represented are the mean ± SD. Each symbol represents one donor. **** adjusted p value of <0.0001. *** adjusted p value of <0.001. * adjusted p value of <0.1.
    Certified Ecotropic Retroviral Packaging Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 2. Functionality of BCMA-specific CAR expressing shRNA CD3ζ. (A) Schematic representation of BCMA-specific CAR-shRNA CD3ζ genetic constructs. CAR, chimeric antigen receptor; LTR, long terminal repeat sequence; ψ, retroviral Psi packaging element; 2A, 2A self-cleaving peptides; shRNA, short hairpin ribonucleic acid; tCD34, truncated CD34; UTR, untranslated regions. (B) Percentage of transduced CD34+ cells (left) or BCMA CAR+ cells (right) among living cells at harvest (day 10) of CAR T-cells generated from 7 healthy donors measured by flow cytometry. (C) IFN-γ secretion in the supernatant upon 24 h co-culture of CAR T-cells generated from 7 healthy donors with multiple myeloma cancer cell line RPMI-8226 (1:1 ratio), as measured by ELISA. (D) Cytotoxic activity was measured using an LDH assay in the supernatant of 24 h co-cultures of CAR T-cells generated from 7 healthy donors with multiple myeloma cancer cell line RPMI-8226 (1:1 ratio). (E) IFN-γ release of BCMA-specific CAR generated from 3 healthy donors in an RPMI-8226 co-culture with or without soluble recombinant BCMA-Fc protein (R&D Systems, 5 µg/mL). Represented are the mean ± SD. Each symbol represents one donor. **** adjusted p value of <0.0001. *** adjusted p value of <0.001. * adjusted p value of <0.1.

    Journal: International journal of molecular sciences

    Article Title: Clinical Proof-of-Concept of a Non-Gene Editing Technology Using miRNA-Based shRNA to Engineer Allogeneic CAR T-Cells.

    doi: 10.3390/ijms26041658

    Figure Lengend Snippet: Figure 2. Functionality of BCMA-specific CAR expressing shRNA CD3ζ. (A) Schematic representation of BCMA-specific CAR-shRNA CD3ζ genetic constructs. CAR, chimeric antigen receptor; LTR, long terminal repeat sequence; ψ, retroviral Psi packaging element; 2A, 2A self-cleaving peptides; shRNA, short hairpin ribonucleic acid; tCD34, truncated CD34; UTR, untranslated regions. (B) Percentage of transduced CD34+ cells (left) or BCMA CAR+ cells (right) among living cells at harvest (day 10) of CAR T-cells generated from 7 healthy donors measured by flow cytometry. (C) IFN-γ secretion in the supernatant upon 24 h co-culture of CAR T-cells generated from 7 healthy donors with multiple myeloma cancer cell line RPMI-8226 (1:1 ratio), as measured by ELISA. (D) Cytotoxic activity was measured using an LDH assay in the supernatant of 24 h co-cultures of CAR T-cells generated from 7 healthy donors with multiple myeloma cancer cell line RPMI-8226 (1:1 ratio). (E) IFN-γ release of BCMA-specific CAR generated from 3 healthy donors in an RPMI-8226 co-culture with or without soluble recombinant BCMA-Fc protein (R&D Systems, 5 µg/mL). Represented are the mean ± SD. Each symbol represents one donor. **** adjusted p value of <0.0001. *** adjusted p value of <0.001. * adjusted p value of <0.1.

    Article Snippet: Briefly, transfection of a certified ecotropic retroviral packaging cell line (Phoenix-eco cells (ATCC®, Manassas, VA, USA)) with the plasmid of interest was performed using calcium phosphate.

    Techniques: Expressing, shRNA, Construct, Sequencing, Retroviral, Generated, Flow Cytometry, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Lactate Dehydrogenase Assay, Recombinant